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1. check that your microscope is plugged in and turned on, and that you…

Question

  1. check that your microscope is plugged in and turned on, and that you are starting at the lowest magnification with the stage farthest away from the lens.
  2. use tweezers to remove a small piece of the red onion membrane (from the inside, or concave, part of the onion) and place the membrane, unfolded, on your glass slide.
  3. use your dropper to add one drop of iodine to your onion membrane.
  4. hold your cover slip at a 45 - degree angle and slowly lower it over the onion membrane, being careful not to produce any air bubbles.
  5. observe the cells at the lowest magnification. you are looking for stretched - out, rectangular cells, forming what looks like a brick wall. you may be able to see a circular nucleus inside some cells. (what is the objective magnification here?________) you may want to change the amount of light by adjusting the diaphragm.
  6. draw your observations below in the \low power\ portion of the table.
  7. increase to the second level of magnification and observe again. (what is the objective magnification here?______ what is the total magnification here?______)
  8. draw your observations below in the \medium power\ portion of the table.
  9. clean up your materials according to your teachers instructions and move on to the second part of the lab.

part 2: animal cells

  1. check that your microscope is plugged in and turned on, and that you are starting at the lowest magnification with the stage farthest away from the lens.
  2. place your prepared cheek cell slide on the microscope stage and observe the cells at the lowest magnification. you are looking for roughly circular cells with a dark blob in the center. (what is the objective magnification here?______ what is the total magnification here?______) you may want to change the amount of light by adjusting the diaphragm.
  3. draw your observations below in the \low power\ portion of the table.
  4. increase to the second level of magnification and observe again. (what is the objective magnification here?______ what is the total magnification here?______)
  5. draw your observations below in the \medium power\ portion of the table.
  6. clean up your materials according to your teachers instructions and move on to the second part of the lab.

data table 1: plant
plant cell: low power
plant cell: medium power

Explanation:

Brief Explanations

This is a lab - activity about observing plant (onion) and animal (cheek) cells under a microscope. The questions ask about objective and total magnifications at different power levels. Typically, a light microscope has an eyepiece magnification of 10x. The low - power objective is usually 4x and the medium - power objective is 10x. Total magnification is the product of eyepiece and objective magnifications.

Answer:

For plant cells at low power:
Objective magnification: 4x
Total magnification: \(10\times4 = 40x\)
For plant cells at medium power:
Objective magnification: 10x
Total magnification: \(10\times10=100x\)
For animal cells at low power:
Objective magnification: 4x
Total magnification: \(10\times4 = 40x\)
For animal cells at medium power:
Objective magnification: 10x
Total magnification: \(10\times10 = 100x\)