QUESTION IMAGE
Question
- check that your microscope is plugged in and turned on, and that you are starting at the lowest magnification with the stage farthest away from the lens.
- use tweezers to remove a small piece of the red onion membrane (from the inside, or concave, part of the onion) and place the membrane, unfolded, on your glass slide.
- use your dropper to add one drop of iodine to your onion membrane.
- hold your cover slip at a 45 - degree angle and slowly lower it over the onion membrane, being careful not to produce any air bubbles.
- observe the cells at the lowest magnification. you are looking for stretched - out, rectangular cells, forming what looks like a brick wall. you may be able to see a circular nucleus inside some cells. (what is the objective magnification here?________) you may want to change the amount of light by adjusting the diaphragm.
- draw your observations below in the \low power\ portion of the table.
- increase to the second level of magnification and observe again. (what is the objective magnification here?______ what is the total magnification here?______)
- draw your observations below in the \medium power\ portion of the table.
- clean up your materials according to your teachers instructions and move on to the second part of the lab.
part 2: animal cells
- check that your microscope is plugged in and turned on, and that you are starting at the lowest magnification with the stage farthest away from the lens.
- place your prepared cheek cell slide on the microscope stage and observe the cells at the lowest magnification. you are looking for roughly circular cells with a dark blob in the center. (what is the objective magnification here?______ what is the total magnification here?______) you may want to change the amount of light by adjusting the diaphragm.
- draw your observations below in the \low power\ portion of the table.
- increase to the second level of magnification and observe again. (what is the objective magnification here?______ what is the total magnification here?______)
- draw your observations below in the \medium power\ portion of the table.
- clean up your materials according to your teachers instructions and move on to the second part of the lab.
data table 1: plant
plant cell: low power
plant cell: medium power
This is a lab - activity about observing plant (onion) and animal (cheek) cells under a microscope. The questions ask about objective and total magnifications at different power levels. Typically, a light microscope has an eyepiece magnification of 10x. The low - power objective is usually 4x and the medium - power objective is 10x. Total magnification is the product of eyepiece and objective magnifications.
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For plant cells at low power:
Objective magnification: 4x
Total magnification: \(10\times4 = 40x\)
For plant cells at medium power:
Objective magnification: 10x
Total magnification: \(10\times10=100x\)
For animal cells at low power:
Objective magnification: 4x
Total magnification: \(10\times4 = 40x\)
For animal cells at medium power:
Objective magnification: 10x
Total magnification: \(10\times10 = 100x\)