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Question
you have 3 questions remaining to reach your goal.
which is the first step in gene cloning?
a the gene is cloned inside the bacterium.
b a plasmid is placed into a bacterium.
c the recombinant vector is placed inside a bacterium.
d a gene or piece of dna is placed into a vector molecule.
Brief Explanations
To determine the first step in gene cloning, we analyze each option:
- Option a: Cloning the gene inside the bacterium is a later step, not the first.
- Option b: Placing a plasmid into a bacterium is part of introducing the vector, but not the initial step of preparing the vector with the gene.
- Option c: Placing the recombinant vector into a bacterium is a subsequent step after the gene is inserted into the vector.
- Option d: The first step in gene cloning is to isolate the gene of interest (or a piece of DNA) and insert it into a vector molecule (like a plasmid) to create a recombinant vector. This is the initial step before introducing it into a host (bacterium).
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d. A gene or piece of DNA is placed into a vector molecule.