Question

why dont we add the enzyme into the master mix? a) the enzyme contains an inhibitor so it will not work b) the reaction will start immediately and our measurement will be inaccurate c) the enzyme takes the most volume and will dilute the reaction d) the master mix cannot contain proteins as they interfere with measurements

Explanation:

Enzymes are biological catalysts. If added to the master - mix prematurely, the reaction will start immediately and may lead to inaccurate measurements as the reaction conditions for measurement may not be properly set up.

Answer:

b) The reaction will start immediately and our measurement will be inaccurate