Question

1. at what temperature do the primers attach to the target sequences and what is this step called?
2. at what temperature does the dna polymerase attach to the primers and synthesize new dna in the 5 to 3 direction and what is this step called?
3. how many cycles on average does it take of heating, cooling, warming to create billions of copies of the target dna sequence so it is enough we can visualize in a gel?
4. if we use 13 sets of primers instead of just one, how does this affect our results - more reliable? less reliable? no difference? why? explain
5. what is template dna and where did we get ours from for the pcr reaction we prepared?
6. we typically use taq dna polymerase. where is this normally found and why do we use it in pcr?
7. who was the guilty suspect and how could you tell this? be specific and draw what the bands in the gel looked like for the crime scene sample and the guilty suspect.

Explanation:

Answer:

50 - 65°C, annealing
72°C, extension
25 - 35 cycles
Less reliable, multiple primers may bind non - specifically
Template DNA is the original DNA strand, source depends on experiment
Thermus aquaticus, heat - stable for PCR cycles