Question

question 6
video 8.5 - micro pipetting by bionetwork revealed common mistakes that can be done when micro
pipetting. which of the following is a micro pipetting mistake?
a press the plunger to the first stop and not the second stop when acquiring your liquid sample.
b go into the liquid first and then press the plunger. in other words, only press the plunger after
you insert the tip into the liquid to be transferred because you want to see bubbles.
c make certain that depress the plunger slowly. you do not want to use the micro pipettor too
quickly.

Explanation:

When using a micro - pipettor, the correct procedure for acquiring liquid is to press the plunger to the second stop when releasing the liquid, but when acquiring (aspirating) the liquid, the plunger should be pressed to the first stop. Pressing it to the first stop only when acquiring is the correct initial step. Option A describes pressing the plunger to the first stop and not the second stop when acquiring, which is incorrect as the first stop is for aspiration and the second stop is for dispensing.

Answer:

A. Press the plunger to the first stop and not the second stop when acquiring your liquid sample.