Question

question 9
how was louis pasteurs swan neck flask experiment significant in the field of microbiology?
it supported the idea of spontaneous generation.
it linked a specific organism to a specific disease.
it helped scientists understand the classification of microorganisms.
it disproved spontaneous generation and led to the germ theory of disease.

question 10
what is a point mutation?
a mutation that involves the insertion of multiple nucleotides
a mutation that changes a single nucleotide base pair
a mutation that affects the entire chromosome
a mutation that involves the deletion of multiple nucleotides

Explanation:

For Question 9:

• Spontaneous generation was the idea that life could arise from non - living matter. Pasteur's experiment showed that when the swan - neck flask prevented dust (and microbes) from entering the broth, no microbial growth occurred. This disproved spontaneous generation. And it was a precursor to the germ theory of disease (which states that many diseases are caused by microorganisms).
• Linking a specific organism to a specific disease was more related to Koch's work.
• Understanding the classification of microorganisms was not the main significance of this experiment.

For Question 10:

• By definition, a point mutation is a mutation that changes a single nucleotide base pair.
• Insertion or deletion of multiple nucleotides is more in the realm of frameshift mutations (if it's not a multiple of 3).
• A mutation affecting the entire chromosome is a chromosomal mutation, not a point mutation.

Answer:

Question 9: It disproved spontaneous generation and led to the germ theory of disease.
Question 10: A mutation that changes a single nucleotide base pair.