Question

question 44
2 points
substrate
capture
antibody
what kind of elisa assay is shown in the diagram?
a indirect
b sandwich
c direct

Explanation:

In a sandwich ELISA, a capture antibody binds the target antigen, then a detection antibody (with enzyme) binds the antigen, forming a "sandwich" (antigen between two antibodies). The diagram shows a capture antibody, antigen (yellow triangle), detection antibody (with enzyme - E - and substrate), matching sandwich ELISA structure. Indirect ELISA uses a secondary antibody against primary antibody, and direct ELISA has enzyme - labeled primary antibody binding antigen directly (no sandwich of two antibodies for antigen).

Answer:

B. Sandwich