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Question
now match the procedure with what it is doing to help isolate the dna from the other materials in the cell. 1. break open the cell a. squish the fruit to a slush 2. dissolve cell membranes b. filter your extract through cheesecloth 3. precipitate the dna (clumps dna) c. mix in a detergent solution 4. separate organelles, broken cell wall, and membranes from proteins, carbohydrates, and dna d. layer cold alcohol over the extract
Brief Explanations
- Squishing the fruit to a slush (A) breaks open the cell as it physically disrupts the cell structure.
- Mixing in a detergent solution (C) dissolves cell membranes as detergents can break down lipid - based membranes.
- Filtering the extract through cheesecloth (B) separates organelles and other large materials from the DNA - containing solution, but doesn't precipitate DNA directly.
- Laying cold alcohol over the extract (D) precipitates the DNA because DNA is insoluble in cold alcohol.
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- A. Squish the fruit to a slush
- C. Mix in a detergent solution
- B. Filter your extract through cheesecloth
- D. Layer cold alcohol over the extract