name & science #: marceha duff date lab # 15: how does exercise affect cellular respiration? pre - lab: 1. what is bromthymol blue (btb) solution? 2. what color change occurs to btb as you slowly exhale into it? 3. after you have an idea of what to do for the lab, on the back of this paper make a prediction graph with appropriate axes labels (remember, this is only an estimation so dont stress about exact values) about what you think will happen. 4. predict how exercise will affect your bodys production of carbon dioxide. 5. in eukaryotes, the kreb cycle takes place within the

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### Question 1
# Brief Explanations:
Bromothymol blue (BTB) is a pH indicator. It is an aqueous solution used to detect changes in pH, often in biological or chemical contexts related to the presence of acids (like carbonic acid from dissolved CO₂) or bases. In the context of cellular respiration and exercise, it's used to detect carbon dioxide (since CO₂ dissolves in water to form carbonic acid, lowering pH).
# Answer:
Bromothymol blue (BTB) is a pH - indicating solution. It is blue in neutral or basic conditions, green in slightly acidic conditions, and yellow in more acidic conditions. It is commonly used to detect the presence and relative amount of carbon dioxide (as CO₂ dissolves in water to form carbonic acid, which lowers pH) in biological (e.g., related to cellular respiration) or chemical experiments.

### Question 2
# Brief Explanations:
When you exhale, you release carbon dioxide (CO₂). The CO₂ dissolves in the BTB solution and reacts with water to form carbonic acid ($\ce{CO_{2} + H_{2}O
ightleftharpoons H_{2}CO_{3}}$). Carbonic acid is an acid, so it lowers the pH of the solution. BTB is a pH indicator that is blue in neutral/basic solutions. As the pH drops (due to the formation of carbonic acid from exhaled CO₂), BTB changes color: first to green (slightly acidic) and then to yellow (more acidic) as the acidity increases.
# Answer:
When you slowly exhale into BTB solution, the carbon dioxide (CO₂) you exhale dissolves in the solution and forms carbonic acid (lowering the pH). Bromothymol blue, which is typically blue in neutral/basic conditions, will change from blue to green and then to yellow as the solution becomes more acidic (due to the increasing amount of carbonic acid formed from the exhaled CO₂).

### Question 4
# Brief Explanations:
Exercise increases the rate of cellular respiration. Cellular respiration is the process by which cells break down glucose (and other molecules) to produce ATP (energy), and carbon dioxide (CO₂) is a by - product of this process (specifically from the Krebs cycle and the conversion of pyruvate to acetyl - CoA). When you exercise, your cells need more energy, so they perform more cellular respiration. This increased rate of cellular respiration leads to a greater production of CO₂ as a waste product. So, exercise (like jogging compared to standing) will increase the body's production of carbon dioxide.
# Answer:
Exercise (such as jogging) will increase the body's production of carbon dioxide. This is because exercise increases the rate of cellular respiration. During cellular respiration, glucose (and other respiratory substrates) is broken down to release energy (in the form of ATP), and carbon dioxide is a by - product of this metabolic process. As the demand for energy (ATP) increases during exercise, more cellular respiration occurs, leading to a greater amount of carbon dioxide being produced.

### Question 5
# Brief Explanations:
In eukaryotic cells, the Krebs cycle (also known as the citric acid cycle or tricarboxylic acid cycle) is a key part of aerobic cellular respiration. It takes place within the matrix of the mitochondria. The mitochondria are the organelles responsible for aerobic respiration in eukaryotes, and the matrix is the innermost compartment of the mitochondrion, which contains the enzymes necessary for the Krebs cycle to occur.
# Answer:
In eukaryotes, the Krebs cycle takes place within the matrix of the mitochondria. The mitochondria are the organelles of eukaryotic cells where aerobic cellular respiration occurs, and the matrix is the fluid - filled space inside the inner mitochondrial membrane that contains the enzymes required for the Krebs cycle.

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