Question

multiple choice question

which gene transfer method involves the uptake of free dna by competent bacterial cells?

transposition
transformation
transduction
conjugation

Explanation:

Define the mechanism of bacterial transformation

Bacterial transformation is the process by which competent bacterial cells bind to and take up naked, free DNA molecules from the surrounding extracellular environment and integrate them into their own genome.

Compare with other horizontal gene transfer methods

• Transposition: The movement of a DNA segment (transposon) from one site to another within a cell's genome.
• Transduction: The transfer of bacterial DNA from a donor to a recipient cell mediated by a bacteriophage (virus).
• Conjugation: The direct transfer of genetic material between two bacterial cells in physical contact, typically via a sex pilus.

Answer:

• Transposition
• Transformation (Correct answer)
• Transduction
• Conjugation