Question

most animal cells exhibit anchorage dependence, which means that in order to divide,
cells must be attached to a substrate or extracellular matrix of a tissue.
all chromosomes must be attached to spindle microtubules.
cell-surface phospholipids must be attached to those of adjoining cells.
nonkinetochore microtubules from opposite poles must overlap and be attached by motor proteins.

Explanation:

Anchorage dependence means that cells need to be attached to a substrate (like the extracellular matrix in tissues) to divide. The other options are about cell - surface phospholipids (not related to anchorage), chromosome - spindle microtubule attachment (related to mitosis but not anchorage), and nonkinetochore microtubules (also related to mitosis structure, not anchorage).

Answer:

cells must be attached to a substrate or extracellular matrix of a tissue.