Question

match the following molecular biology tool to the appropriate example.

1. restriction enzyme

ecori which cuts at the gaattc/cttaag recognition site.

1. plasmid dna

sample with fragments of specific sizes such as 0.5kb, 1kb, 2kb, 3kb and 4kb

1. molecular weight marker

able to dispense down to 1 microliter

1. micropipettes

puc19 found in e. coli

Explanation:

• Micro - pipettes (4) are tools used to dispense small volumes like microliters.
• Plasmid DNA (2) such as pUC19 (found in E.coli) is a type of DNA molecule.
• Restriction Enzymes (1) like EcoRI have specific recognition sites (e.g., GAATTC/CTTAAG) and cut DNA.
• Molecular weight markers (3) come in specific sizes (e.g., 0.5Kb, 1Kb, 2Kb, 3Kb, 4Kb) and are used to estimate the size of DNA fragments in a sample.

Answer:

1. Restriction Enzyme - EcoRI which cuts at the GAATTC/CTTAAG recognition site.
2. Plasmid DNA - pUC19 found in E. coli.
3. Molecular weight marker - Sample with fragments of specific sizes such as 0.5Kb, 1Kb, 2Kb, 3Kb and 4Kb.
4. Micropipettes - Able to dispense down to 1 microliter.