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once a container of medium has been inoculated, the next step of the five ts involves placing the medium in a temperature - controlled chamber to encourage multiplication.
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The Five Ts in microbiology (or related fields) include "Incubation" as a step where after inoculation, the medium is placed in a temperature - controlled environment (like an incubator) to promote microbial multiplication. The context here is about a step after inoculation involving temperature - controlled chambers for multiplication, so the term is "Incubation".
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Incubation