Question

37 multiple choice 1 point for agarose electrophoresis to work all the following must be true except, dna has negative charges. agarose gels have pores. tbe buffer has ions. ions in tbe buffer flows from positive pole to negative pole because they have negative charges.

Explanation:

To solve this, we analyze each option:

1. DNA has negative charges: True, as phosphate groups in DNA give it negative charge, essential for moving towards positive electrode in electrophoresis.
2. Agarose gels have pores: True, pores allow separation of DNA fragments based on size.
3. TBE buffer has ions: True, ions in buffer conduct electricity, necessary for electrophoresis.
4. Ions in TBE buffer flows from positive pole to negative pole because they have negative charges: False. Negative ions move to positive pole, positive ions to negative pole. So this statement is incorrect.

Answer:

D. Ions in TBE buffer flows from positive pole to negative pole because they have negative charges.