Question

1. if you were trying to come up with an easy-to-remember analogy for the polymerase chain reaction, you would likely say pcr is similar to a

o dishwasher
o colored pencils
o photocopier
o scissors

1. what determines the piece of dna that is amplified in pcr?

o the specific restriction enzyme used
o the specific primers used
o the temperature of each cycle
o the source of the dna

1. what is the role of temperature in pcr?

o it is used to enhance building of the dna chains
o it is required for the use of restriction enzymes
o it is used to separate and anneal the nucleic acids

Explanation:

1. PCR (Polymerase Chain Reaction) is a technique used to amplify (make many copies of) a specific segment of DNA. A photocopier also makes multiple copies of a document. So, PCR is similar to a photocopier.
2. In PCR, primers are short, single - stranded DNA sequences that are complementary to the regions flanking the target DNA segment. The specific primers used determine which piece of DNA is amplified.
3. In PCR, different temperatures are used for different steps. High temperature is used to separate (denature) the double - stranded DNA into single strands. Then, a lower temperature is used for annealing (where primers bind to the single - stranded DNA).

Answer:

1. photocopier
2. The specific primers used
3. It is used to separate and anneal the nucleic acids