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Question
you better add a buffer quickly so that the neurons dont dry out! what is an important characteristic of the buffer used for studying sodium in patch clamp experiments?
a) one that blocks potassium and calcium channels
b) one that blocks sodium channels
c) one that blocks chloride channels
d) one that blocks sodium and potassium channels
In patch - clamp experiments studying sodium, to isolate sodium currents, other ion channels (like potassium and calcium channels) need to be blocked. If potassium and calcium channels are not blocked, their currents would interfere with the measurement of sodium - specific currents. Blocking sodium channels (option b) would defeat the purpose of studying sodium. Blocking chloride channels (option c) is not relevant to studying sodium. Blocking sodium channels along with potassium (option d) is incorrect as we want to study sodium.
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A. One that blocks potassium and calcium channels