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Question
which situation would lead to degradation of dna in a sample?
○ improperly stored samples
○ contaminated samples
○ not enough sample material (i.e., cells)
○ use of a centrifuge
○ use of chemicals to separate dna from other cell parts
Improper storage (e.g., high temperature, exposure to nucleases) can break DNA strands. Contamination with nucleases (enzymes degrading DNA) from external sources also causes DNA degradation. Insufficient sample affects yield, not degradation; centrifugation and chemical separation are standard steps that do not inherently degrade DNA.
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A. improperly stored samples, B. contaminated samples