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Question
which of the following types of information would be most useful in an effort to move a dna fragment from one plasmid vector to another, using molecular cloning techniques? multiple choice dna fingerprint data restriction maps of the plasmids sequence of pcr primers data from cloning of dolly the sheep
Restriction maps show the locations of restriction - enzyme recognition sites in the plasmids. This information is crucial for cutting the DNA fragment from the source plasmid and the target plasmid at appropriate sites for ligation during molecular cloning. DNA fingerprint data is more for identification purposes, PCR primers are for amplification not for moving fragments between plasmids directly, and data from Dolly the sheep cloning is not relevant to this specific molecular cloning task of moving a DNA fragment between plasmids.
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B. Restriction maps of the plasmids