Question

which of the following is not a type of cloning vector used to transfer recombinant dna into a cloning host?
plasmids
bacteriophages
cosmids
e. coli
question 8 (1 point)
a quick way to determine whether bacterial colonies have incorporated a gene of interest is
to apply the sanger method.
to perform an rflp.
to do a hybridization test.
to make a genomic library.
question 9 (1 point)
azoles are used to fight malarial parasites.
true
false
question 10 (1 point)
the kirby-bauer test identifies agents that can be used to fight specific bacteria.
true
false

Explanation:

• Question 7: Cloning vectors are DNA molecules that can carry foreign DNA into a host cell. Plasmids, bacteriophages, and cosmids are all types of cloning vectors. E. coli is a bacterium that can be used as a cloning host, not a vector.
• Question 8: Hybridization tests, such as Southern blotting, can quickly determine if bacterial colonies have incorporated a gene of interest by using a labeled probe that binds to the specific DNA sequence. The Sanger method is for DNA sequencing, RFLP (Restriction Fragment Length Polymorphism) is for analyzing DNA fragments based on restriction enzyme digestion, and making a genomic library is for storing an organism's DNA.
• Question 9: Azoles are antifungal agents, not used to fight malarial parasites. Antimalarial drugs include chloroquine, artemisinin - based combinations, etc.
• Question 10: The Kirby - Bauer test (disk diffusion test) is used to determine the susceptibility of bacteria to various antimicrobial agents. It helps identify which agents can be used to fight specific bacteria.

Answer:

• Question 7: E. coli
• Question 8: to do a hybridization test
• Question 9: False
• Question 10: True