Question

what is the appropriate biosafety level for an organism like mycobacterium tuberculosis that can be deadly and can transfer between people through the air but has established effective treatments?
bsl1
bsl2
bsl3
bsl4
what is the correct wavelength to measure bacteria with spectrometry?*
400 nm
500 nm
600 nm
700 nm
what factor distinguishes between a bsl2 and bsl3 organism?*
eukaryotic vs prokaryotic
whether the disease transmits through the air or physical contact
how recently the organism was documented in peer reviewed literature
how effective treatments for the disease are

Explanation:

• First Question: Mycobacterium Tuberculosis is a pathogen that can be transmitted through the air (aerosol - transmissible) and is potentially deadly. Biosafety Level 3 (BSL3) is used for agents that can cause serious or potentially lethal disease through inhalation. Although there are treatments, the mode of transmission (air - borne) and the severity of the disease (deadly) classify it as BSL3. BSL1 is for non - pathogenic agents, BSL2 for agents that cause moderate disease with limited transmission, and BSL4 for highly dangerous agents with no treatments.
• Second Question: A wavelength of 600 nm is commonly used in spectrometry to measure bacteria. At this wavelength, there is sufficient light absorption by bacterial cells (due to their cellular components) to accurately measure turbidity (a measure of bacterial concentration). 400 nm and 500 nm may be affected by other cellular components (like pigments in some bacteria) that can interfere with the measurement, and 700 nm may have less absorption by bacterial cells compared to 600 nm.
• Third Question: One of the key differences between BSL2 and BSL3 organisms is the mode of transmission. BSL3 organisms are often aerosol - transmissible (transmit through the air), while BSL2 organisms are usually transmitted through physical contact (e.g., direct contact with body fluids). The distinction is not based on eukaryotic vs prokaryotic (both can be in different BSL levels), how recently they were documented (not a safety - related factor), or treatment effectiveness (BSL3 can have treatments as in the case of tuberculosis).

Answer:

• First Question: BSL3
• Second Question: 600 nm
• Third Question: Whether the disease transmits through the air or physical contact