Question

video links
safeshare.tv link: https://safeshare.tv/1vcm
original youtube video: https://youtu.be/lseygwup6ko?1=106

dna sequence #1
dna: t a c t a a c g c c c c t c t t a c a a a a t a a t t
mrna:
aa:

dna sequence #2
dna: a a t a c g c c c g c g t a t t g t t t a g a a t t c
mrna:
aa:

dna sequence #3
dna: t t t a t t a c c a c c t g c c t a g t c a g a c t t
mrna:
aa:

Explanation:

Step1: Transcribe DNA to mRNA (DNA Sequence #1)

In transcription, DNA's T pairs with A, A with U, C with G, G with C. DNA: TACTAACGCCCCCTCTTACAAAATAATT. So mRNA: AUGAUUGCGGGGAGAAUGUUUUUAUUAA.

Step2: Translate mRNA to AA (DNA Sequence #1)

Use codon table. AUG (Met), AUU (Ile), GCG (Ala), GGG (Gly), AGA (Arg), AUG (Met), UUU (Phe), UUA (Leu), UAA (stop). So AA: Met - Ile - Ala - Gly - Arg - Met - Phe - Leu - (stop).

Step3: Transcribe DNA to mRNA (DNA Sequence #2)

DNA: AATACGCCCCGCGTATTGTTTAGAATTC. mRNA: UUAUGCGGGGCGCAUUAACAAAUCUUAAG.

Step4: Translate mRNA to AA (DNA Sequence #2)

UUA (Leu), AUG (Met), CGC (Arg), GGG (Gly), CGC (Arg), AUU (Ile), AAC (Asn), AAA (Lys), UCU (Ser), UAA (stop). AA: Leu - Met - Arg - Gly - Arg - Ile - Asn - Lys - Ser - (stop).

Step5: Transcribe DNA to mRNA (DNA Sequence #3)

DNA: TTTATTACCACCTGCCCTAGTCAGACTT. mRNA: AAAUAAUGGUGGACGGGGAU CAGUCUGAA.

Step6: Translate mRNA to AA (DNA Sequence #3)

AAA (Lys), UAA (stop), UGG (Trp), UGG (Trp), ACG (Thr), GGG (Gly), GAU (Asp), CAG (Gln), UCU (Ser), GAA (Glu). But UAA is stop, so AA: Lys - (stop) - Trp - Trp - Thr - Gly - Asp - Gln - Ser - Glu (note: start from first AUG? Wait, maybe I missed AUG. Wait DNA #3: TTTATTACCACCTGCCCTAGTCAGACTT. Let's re - transcribe: T→A, T→A, T→A, A→U, T→A, A→U, C→G, C→G, A→U, C→G, C→G, T→A, G→C, C→G, C→G, T→A, A→U, G→C, T→A, C→G, A→U, G→C, A→U, C→G, T→A, T→A. Wait maybe better to group into codons. DNA #3: TTT ATT ACC ACC TGC CCT AGT CAG ACT T. mRNA: AAA UAA UGG UGG ACG GGG GAU CAG UCU GAA. Now codons: AAA (Lys), UAA (stop), UGG (Trp), UGG (Trp), ACG (Thr), GGG (Gly), GAU (Asp), CAG (Gln), UCU (Ser), GAA (Glu). But since UAA is stop, translation stops at UAA. But maybe the start codon is AUG? Wait no AUG in mRNA? Wait DNA #3: Did I transcribe wrong? DNA: TTTATTACCACCTGCCCTAGTCAGACTT. Let's write each base: T T T A T T A C C A C C T G C C C T A G T C A G A C T T. So mRNA: A A A U A A U G G U G G A C G G G G A U C A G U C U G A A. Now codons: AAA (Lys), UAA (stop), UGG (Trp), UGG (Trp), ACG (Thr), GGG (Gly), GAU (Asp), CAG (Gln), UCU (Ser), GAA (Glu). So AA sequence: Lys, (stop), Trp, Trp, Thr, Gly, Asp, Gln, Ser, Glu.

Answer:

DNA Sequence #1

• mRNA: AUGAUUGCGGGGAGAAUGUUUUUAUUAA
• AA: Met - Ile - Ala - Gly - Arg - Met - Phe - Leu - (stop)

DNA Sequence #2

• mRNA: UUAUGCGGGGCGCAUUAACAAAUCUUAAG
• AA: Leu - Met - Arg - Gly - Arg - Ile - Asn - Lys - Ser - (stop)

DNA Sequence #3

• mRNA: AAAUAAUGGUGGACGGGGAUCAGUCUGAA
• AA: Lys - (stop) - Trp - Trp - Thr - Gly - Asp - Gln - Ser - Glu