Question

1. using the following dna sequence as a template and the complementary base pair rule, write the complementary sequence that would be found on the opposite dna strand. 5 a-t-c-c-a-t-c-g-a-c-t-a-a-t-a-c-c-g-a-c-c-g-a-t-3

Explanation:

Step1: Recall base - pairing rules

In DNA, adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G).

Step2: Write complementary bases for each base in the given sequence

Given the sequence \(5'\text{-A-T-C-C-A-T-C-G-A-C-T-A-A-T-C-G-A-C-C-G-A-T - 3}'\), for each base:

• A (in the given strand) will pair with T.
• T (in the given strand) will pair with A.
• C (in the given strand) will pair with G.
• G (in the given strand) will pair with C.

The complementary sequence will be in the \(3'\) to \(5'\) direction. So, flipping the direction and applying base - pairing:
The complementary sequence is \(3'\text{-T-A-G-G-T-A-G-C-T-G-A-T-T-A-G-C-T-G-G-C-T-A - 5}'\)

Answer:

\(3'\text{-T-A-G-G-T-A-G-C-T-G-A-T-T-A-G-C-T-G-G-C-T-A - 5}'\)