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Question
share out/discussion (5 minutes)
- the proportions of the bases are consistent within a species; however they do vary between species.
using the base - pair rules, complete the following table to show the percentage of each type of base in the five different organisms.
Step1: Apply Chargaff's rule
According to Chargaff's rule, in DNA, \(A = T\) (adenine equals thymine) and \(G = C\) (guanine equals cytosine), and \(A+T + G + C=100\%\).
Step2: Calculate for Wheat
For wheat, since \(A = 27\%\), then \(T=27\%\). So \(G + C=100-(27 + 27)=46\%\). Since \(G = C\), then \(G = C=\frac{46}{2}=23\%\).
Step3: Calculate for Salmon
For salmon, \(G + C=100-(29 + 21)=50\%\). Since \(G = C\), then \(G = C = 25\%\).
Step4: Calculate for Cow
For cow, since \(A = 28\%\), then \(T = 28\%\). So \(G + C=100-(28+28)=44\%\). Since \(G = C\), then \(G = C=\frac{44}{2}=22\%\).
Step5: Calculate for Yeast
For yeast, since \(A = 31\%\), then \(T = 31\%\). So \(G + C=100-(31 + 31)=38\%\). Since \(G = C\), then \(G = C=\frac{38}{2}=19\%\).
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| Organism | Adenine | Guanine | Cytosine | Thymine |
|---|---|---|---|---|
| Wheat | 27 | 23 | 23 | 27 |
| Salmon | 21 | 25 | 25 | 29 |
| Cow | 28 | 22 | 22 | 28 |
| Human | 31 | 19 | 19 | 31 |