Question

question 41
2 points
is a secondary antibody needed for this example?
a yes
b no

Explanation:

In antibody - related assays (like immunoassays), a secondary antibody is used to detect the primary antibody. The diagram shows a setup where a secondary - like interaction (with the red antibody interacting with the green antibody - antigen complex) is occurring. If we analyze the typical immunoassay workflow, when we have a primary antibody (green, binding to the antigen) and then a secondary antibody (red, binding to the primary antibody) to amplify or detect the signal, in this case, the presence of the red antibody (acting as secondary) - primary (green) interaction implies that a secondary antibody is needed. So the answer is Yes.

Answer:

A. Yes