Question

question 23 (1 point) saved
in hybridization, oligonucleotides function as
restriction enzymes.
vectors.
ligases.
probes.
question 24 (1 point) saved
dna replication involves all these steps except
unzipping hydrogen bonds between complementary base pairs.
dissolution of the nuclear membrane.
synthesizing new dna matching complementary base pairs.
uncoiling parent dna.
question 25 (1 point) saved
the three major side effects of antimicrobials are toxicity to organs, allergic reactions, and suppression or alteration of blood cells.
true
false

Explanation:

• Question 23: In hybridization, oligonucleotides function as probes. Probes are short, single - stranded DNA or RNA sequences that can bind to complementary sequences in a target nucleic acid. Restriction enzymes cut DNA at specific sequences, vectors are used to carry foreign DNA (e.g., plasmids), and ligases join DNA fragments.
• Question 24: DNA replication does not involve the dissolution of the nuclear membrane. DNA replication steps include uncoiling the parent DNA (by helicase - like activities in some organisms), unzipping hydrogen bonds between complementary base pairs (helicase function), and synthesizing new DNA matching complementary base pairs (DNA polymerase function). The nuclear membrane dissolution is part of processes like mitosis or meiosis in eukaryotes, not specifically DNA replication.
• Question 25: The three major side effects of antimicrobials are toxicity to organs (e.g., nephrotoxicity from some antibiotics), allergic reactions (common with penicillins), and suppression or alteration of blood cells (e.g., some drugs can cause anemia). So the statement is True.

Answer:

• Question 23: probes
• Question 24: dissolution of the nuclear membrane
• Question 25: True