Question

procedure part 2:

1. place one chunk of potato in the bottom of three different test tubes; the fourth one should be a cooked potato.
2. place 5 ml of an acid solution in one test tube, 5 ml of water in a second test tube, 5 ml of a basic solution in the third, test tube. find the ph of each solution and record it into your data table.
3. wait 5 minutes.
4. add 5 ml of hydrogen peroxide to the solution in each tube and record the relative amount of bubbling you see in each. decide how the data will be collected, quantitatively.

results/data:
create a data table with appropriate title and headings for this experiment on a separate sheet of paper. remember to identify your constants, number of trials, average, and manipulated/responding variables.
analyze data:
make the appropriate graph of the data you collected. remember title, labels, and units of measurement. d-tails & dry mix! insert the graph at the bottom of the document.
conclusion:

1. how did you know that the catalase was working?________________________
2. if catalase is working properly, what is the liquid substance that is left inside the test tube at the end of the reaction time? explain by analyzing the chemical equation of the reaction.

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1. most enzymes in living systems function optimally at a ph of 7.0. did the results of our lab support these observations? explain why or why not completely.________________________

Explanation:

1. Catalase breaks down hydrogen peroxide into water and oxygen. The oxygen is released as bubbles. So, the presence of bubbling indicates that catalase is working.
2. The chemical equation for the reaction catalyzed by catalase is \(2H_{2}O_{2}\xrightarrow{\text{catalase}}2H_{2}O + O_{2}\uparrow\). As we can see from the equation, when catalase works properly, the liquid substance left is water (\(H_{2}O\)) because hydrogen peroxide (\(H_{2}O_{2}\)) is decomposed into water and oxygen (\(O_{2}\), which is a gas).
3. If in the lab, the test tube with water (which has a pH close to 7) showed the most bubbling (indicating the highest enzyme activity), then the results support the observation that most enzymes function optimally at a pH of 7.0. If another solution (acidic or basic) showed more bubbling, then the results do not support this observation.

Answer:

1. The presence of bubbling.
2. Water (\(H_{2}O\)), because \(2H_{2}O_{2}\xrightarrow{\text{catalase}}2H_{2}O + O_{2}\uparrow\).
3. If the water - containing test tube (pH≈7) had the most bubbling: Yes, because it showed the highest enzyme (catalase) activity at pH≈7. If not: No, because another pH (acidic or basic) showed higher enzyme activity.