Question

most animal cells exhibit anchorage dependence, which means that in order to divide,
cell - surface phospholipids must be attached to those of adjoining cells.
nonkinetochore microtubules from opposite poles must overlap and be attached by motor proteins.
cells must be attached to a substrate or extracellular matrix of a tissue.
all chromosomes must be attached to spindle microtubules.

Explanation:

Anchorage dependence in animal cells means cells need to be attached to a substrate (like the extracellular matrix of a tissue) to divide. The first option about phospholipids is incorrect as it's about cell - cell attachment not anchorage for division. Nonkinetochore microtubules and chromosome - spindle attachment are related to mitosis mechanics but not the anchorage dependence concept. The correct idea is cells attaching to a substrate or extracellular matrix.

Answer:

cells must be attached to a substrate or extracellular matrix of a tissue.