QUESTION IMAGE
Question
the lamin b receptor is found in the inner membrane of the nuclear envelope. it connects the nuclear envelope to the heterochromatin (chromosome) and nuclear lamina proteins, which provide structure to the nucleus. in normal cells, the lamin b receptor protein is stably locked in place by these interactions and shows very little movement. infection of cells by the herpes simplex virus type i (hsv - 1) can disrupt the lamin b receptor interactions when the virus capsids exit from the nucleus by budding through the inner nuclear membrane of the nucleus. this causes some of the lamin b receptor to move between the inner nuclear membrane and the er membrane.
frap was completed for lamin b or a control protein under different conditions. match the three cellular treatments with the correct frap graph.
(a) lamin b receptor in virus - infected cells
(b) lamin b receptor in normal cells
(c) control protein known to cycle rapidly between the inner nuclear membrane and other cellular membranes
In normal cells, lamin B receptor has little movement, so its FRAP graph will show little fluorescence recovery (graph A). In virus - infected cells, some lamin B receptor moves, so there will be some fluorescence recovery (graph B). The control protein that rapidly cycles will show a high degree of fluorescence recovery (graph C).
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A. Lamin B receptor in normal cells - Graph A
B. Lamin B receptor in virus - infected cells - Graph B
C. Control protein known to cycle rapidly - Graph C