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Question
dna replication
learning objective 19.2: given a diagram of a dna molecule during replication, label the following: the origin of replication, directions of replication, replication fork, the leading strand, replisome.
drag and drop the events that occur during dna replication into the correct chronological order. place the first event at the top and the last event at the bottom.
- rna primers are added.
- dna polymerase adds matching base pairs to template strands.
- rna primers are removed.
- an enzyme connects nucleotides at each gap on the lagging strand.
- replication forks are formed.
Step1: Recall DNA replication steps
DNA replication starts with the formation of replication forks (initiation). Then RNA primers are added to provide a starting point for DNA synthesis. Next, DNA polymerase adds complementary base pairs. After that, RNA primers are removed. Finally, an enzyme (like DNA ligase) connects the gaps on the lagging strand.
Step2: Order the events
- Replication forks are formed (initiation of replication).
- RNA primers are added (to initiate DNA synthesis).
- DNA polymerase adds matching base pairs to template strands (elongation).
- RNA primers are removed (to replace with DNA).
- An enzyme connects nucleotides at each gap on the lagging strand (ligation, specifically for Okazaki fragments on lagging strand).
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- Replication forks are formed.
- RNA primers are added.
- DNA polymerase adds matching base pairs to template strands.
- RNA primers are removed.
- An enzyme connects nucleotides at each gap on the lagging strand.