Question

your capsule stain is complete and correct. which of the following statements would apply to the image you see?

select all that apply.

a. you can observe encapsulated organisms in this field.
b. the capsules have stained with the primary and secondary dye colors.
c. the background is stained dark with nigrosin.
d. no bacterial capsules can be visualized in this field.
e. the bacterial cells are stained with crystal violet.

Explanation:

Analyze capsule stain principles

A capsule stain is a combination of a negative stain (to color the background) and a positive simple stain (to color the bacterial cell body). Because bacterial capsules are non-ionic and water-soluble, they do not bind to basic or acidic dyes and remain unstained, appearing as clear halos surrounding the colored bacterial cells.

Evaluate each statement

• A. You can observe encapsulated organisms in this field.

True. A complete and correct capsule stain allows the visualization of encapsulated organisms, which appear as cells surrounded by clear halos.

• B. The capsules have stained with the primary and secondary dye colors.

False. Capsules do not stain; they remain colorless/clear.

• C. The background is stained dark with nigrosin.

True. Nigrosin (or India ink) is an acidic, negatively charged dye used to stain the background dark in a capsule stain.

• D. No bacterial capsules can be visualized in this field.

False. Since the stain is complete and correct, capsules are successfully visualized as clear halos.

• E. The bacterial cells are stained with crystal violet.

True. Crystal violet (or copper sulfate/safranin depending on the specific method, but crystal violet is the standard primary basic stain in Anthony's capsule staining method) is used to stain the vegetative bacterial cells inside the capsule.

Answer:

• A. You can observe encapsulated organisms in this field. (Correct answer)
• B. The capsules have stained with the primary and secondary dye colors.
• C. The background is stained dark with nigrosin. (Correct answer)
• D. No bacterial capsules can be visualized in this field.
• E. The bacterial cells are stained with crystal violet. (Correct answer)