Question

question 6
what are the main steps of pcr?

1. after one cycle, dna molecules are doubled
2. denaturation of the dna at 94 to 98 oc
3. primer annealing at 55 to 65 oc
4. primer extension at 72 oc

Explanation:

PCR (Polymerase Chain Reaction) has three main steps. Denaturation separates DNA strands. Primer annealing allows primers to bind. Primer extension synthesizes new DNA strands. After cycles, DNA doubles.

Answer:

The main steps of PCR are:

1. Denaturation of the DNA at 94 to 98°C (separates double - stranded DNA into single strands).
2. Primer annealing at 55 to 65°C (primers bind to complementary sequences on the single - stranded DNA).
3. Primer extension at 72°C (DNA polymerase synthesizes new DNA strands complementary to the template strands). After each cycle, the number of DNA molecules is doubled.