Question

multiple choice question
in rt - qpcr, ________.
o the amount of cdna produced during the rt step is measured in real time
o the amount of rna used as a template during the rt step is measured in real time
o the amount of dna produced during the pcr step is measured in real time
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Explanation:

RT - qPCR stands for Reverse Transcription - Quantitative Polymerase Chain Reaction. In RT - qPCR, first, RNA is reverse - transcribed into cDNA (Reverse Transcription step). Then, the cDNA is amplified using PCR. The key feature of qPCR (the quantitative part) is that the amount of DNA produced during the PCR step is measured in real - time. This is usually done by using fluorescent dyes or probes that bind to the DNA as it is being synthesized. Measuring the amount of cDNA during the RT step (first option) is not the real - time measurement part of RT - qPCR. Measuring the amount of RNA (second option) is also not the real - time measurement step. The real - time measurement occurs during the PCR amplification of the cDNA (which is DNA).

Answer:

the amount of DNA produced during the PCR step is measured in real time