Question

most animal cells exhibit anchorage dependence, which means that in order to divide,

nonkinetochore microtubules from opposite poles must overlap and be attached by motor proteins.

cell - surface phospholipids must be attached to those of adjoining cells.

cells must be attached to a substrate or extracellular matrix of a tissue.

all chromosomes must be attached to spindle microtubules.

Explanation:

Anchorage dependence means that cells need to be attached to a surface to divide. Nonkinetochore microtubules and chromosome - spindle attachment are related to mitosis mechanics. Cell - surface phospholipid attachment is not the key for anchorage dependence. The correct concept is that cells must attach to a substrate or extracellular matrix.

Answer:

cells must be attached to a substrate or extracellular matrix of a tissue.