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after incubation in tryptone broth, what reagent is added to test for i…

Question

after incubation in tryptone broth, what reagent is added to test for indole production?
iodine
hydrogen peroxide
methyl red
kovacs reagent
question 6 (1 point)
phenol red in the phenol red broth is a(n)_______________.
end product
carbohydrate
reagent
ph indicator

Explanation:

Brief Explanations
  • For the first question: Kovac's reagent is used to test for indole production. It reacts with indole (if present) to form a red - colored complex. Iodine is used in tests like the starch - iodine test. Hydrogen peroxide is used in catalase tests. Methyl red is used in the methyl red test for pH changes related to mixed acid fermentation.
  • For the second question: Phenol red in phenol red broth is a pH indicator. It changes color depending on the pH of the broth. An end product would be something like an acid or gas produced by bacterial metabolism. A carbohydrate is a nutrient source in the broth. A reagent is a general term for a chemical used in a test, but more specifically, phenol red's role is as a pH - sensitive color - changing substance.

Answer:

  • First question: Kovac's reagent
  • Second question: pH indicator