Question

what are you trying to define when you ask the question \what is my assignment about?\
the audience
the topic
the research
the resources

Explanation:

When asking "What is my assignment about?", one is seeking to understand the subject matter or focus of the assignment, which is the topic. "The audience" refers to who the assignment is for, "the research" is about investigating, and "the resources" are materials used; none match the question's intent.

Answer:

the topic