Question

1. which of following factors has the greatest impact on an enzyme?

• concentration of substrate
• temperature
• ph
• concentration of enzymes

Explanation:

Enzymes are proteins, and their structure (and thus function) is highly sensitive to temperature. High temperatures can denature enzymes (alter their 3 - D structure), making them non - functional. While substrate concentration affects reaction rate (more substrate can increase rate until saturation), it does not permanently damage the enzyme. pH also affects enzyme function (each enzyme has an optimal pH), but temperature (especially extreme heat) can cause more irreversible damage. Enzyme concentration affects the rate of reaction (more enzyme can increase rate if substrate is available), but it does not change the enzyme's structure in the same way as temperature.

Answer:

temperature