QUESTION IMAGE
Question
transcribe and translate both the original and mutated dna strand below. what kind of mutation occurred did any changes occur to the amino acids produced?
original dna: tacgaattttcggcaattag
mutated dna: tacgaattttccgcaattag
answer:
Step1: Transcribe Original DNA
Transcription: DNA → mRNA. Replace T with U.
Original DNA: TACGAATTT CGGCAATTAG
mRNA: AUGCUUAAA GCCGUUAAUC
Step2: Translate Original mRNA
Translation: mRNA → amino acids (using codon table).
AUG - Methionine, CUU - Leucine, AAA - Lysine, GCC - Alanine, GUU - Valine, AUC - Isoleucine
Amino acids: Methionine - Leucine - Lysine - Alanine - Valine - Isoleucine
Step3: Transcribe Mutated DNA
Mutated DNA: TACGAATTT CCGCAATTAG
mRNA: AUGCUUAAA GGC GUUAAUC
Step4: Translate Mutated mRNA
AUG - Methionine, CUU - Leucine, AAA - Lysine, GGC - Glycine, GUU - Valine, AUC - Isoleucine
Amino acids: Methionine - Leucine - Lysine - Glycine - Valine - Isoleucine
Step5: Identify Mutation
Compare original (CGG) and mutated (CCG) codons.
It is a point mutation (single - base substitution).
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Original DNA transcription: AUGCUUAAA GCCGUUAAUC; translation: Methionine - Leucine - Lysine - Alanine - Valine - Isoleucine.
Mutated DNA transcription: AUGCUUAAA GGC GUUAAUC; translation: Methionine - Leucine - Lysine - Glycine - Valine - Isoleucine.
Mutation: Point mutation (single - base substitution). Amino acid change: Alanine (from original) → Glycine (from mutated).