Question

staining • spore staining
question
based on what you understood from the video, which of the following statements are correct?
select all that apply.
a. in this image, the endospores are green in color.
b. in this image, the endospores are red in color.
c. this image could be representative of a bacillus species.
d. this image shows primarily exospores.

Explanation:

Analyze the staining results in the image

The image shows a classic endospore stain (Schaeffer-Fulton method) where vegetative bacterial cells are stained red/pink by safranin, and the endospores (both inside the cells and free) are stained green by malachite green.

Evaluate the taxonomic representation

The bacteria shown are Gram-positive, rod-shaped bacilli arranged in chains, containing distinct green endospores. This morphology and spore-forming capability are highly characteristic of the genus Bacillus (such as Bacillus anthracis or Bacillus cereus).

Assess the options

• A is correct: The endospores are clearly visible as green oval structures.
• B is incorrect: The vegetative cells are red, not the endospores.
• C is correct: The rod-shaped, spore-forming morphology is representative of Bacillus species.
• D is incorrect: The structures shown are endospores (formed intracellularly), not exospores.

Answer:

• A. In this image, the endospores are green in color. (Correct answer)
• B. In this image, the endospores are red in color.
• C. This image could be representative of a Bacillus species. (Correct answer)
• D. This image shows primarily exospores.