Question

staining • spore staining
laboratory simulation
steps
apply malachite green and a small strip of paper covering to a prepared smear
steam over heat source
apply safranin to smear
rinse
rinse and blot dry

Explanation:

Identify the correct order of the Schaeffer-Fulton endospore staining protocol

The standard steps for the endospore stain (Schaeffer-Fulton method) are:

1. Apply malachite green (primary stain) and a small strip of paper covering to a prepared smear.
2. Steam over a heat source (mordant to force the stain into the endospore).
3. Rinse with water (decolorizer to wash the primary stain from vegetative cells).
4. Apply safranin to the smear (counterstain for vegetative cells).
5. Rinse and blot dry.

Map the given steps to their correct sequential positions

• Step 1: Apply malachite green and a small strip of paper covering to a prepared smear
• Step 2: Steam over heat source
• Step 3: Rinse
• Step 4: Apply safranin to smear
• Step 5: Rinse and blot dry

Answer:

No.	Answer
2	Steam over heat source
3	Rinse
4	Apply safranin to smear
5	Rinse and blot dry