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Question
in reverse transcription pcr, ________. mrna is used to produce millions of copies of its complementary dna millions of rna copies are produced from a specific mrna sequence dna is used to produce millions of copies of complementary rna
Reverse transcription PCR (RT - PCR) involves using mRNA as a template. The first step is reverse transcription, where mRNA is converted into complementary DNA (cDNA) by reverse transcriptase. Then, PCR is used to amplify this cDNA, resulting in millions of copies of the cDNA.
- The second option is incorrect because RT - PCR produces DNA (cDNA) copies, not RNA copies.
- The third option is incorrect as it is the reverse of what happens in RT - PCR (DNA is not the starting material to produce RNA in RT - PCR; mRNA is the starting material to produce DNA).
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mRNA is used to produce millions of copies of its complementary DNA.