Question

question 1 (1 point)
the kligers iron agar tests differentiate bacteria based on their ability to:
produce endospores
produce antibiotics
ferment sugars and reduce sulfur
hydrolyze lipids
question 2 (1 point)
which of the following tests involves the enzyme catalase?
nitrate reduction test
catalase test
gelatin hydrolysis
starch hydrolysis

Explanation:

Question 1

Kliger's Iron Agar contains glucose, lactose, and ferrous sulfate. Bacteria that ferment sugars (glucose and/or lactose) will change the pH (affecting the phenol red indicator). Sulfur reduction (by bacteria) reacts with ferrous sulfate to form black FeS. Endospore production is tested by spore - forming assays, antibiotic production is a different metabolic trait assay, and lipid hydrolysis is tested by lipid - containing media (e.g., spirit blue agar for lipid hydrolysis).

The Catalase Test is specifically designed to detect the presence of the catalase enzyme. Catalase converts hydrogen peroxide ($H_2O_2$) to water ($H_2O$) and oxygen ($O_2$) (the reaction is $2H_2O_2
ightarrow2H_2O + O_2$). The Nitrate Reduction Test is for nitrate - reducing bacteria (involves nitrate reductase), Gelatin Hydrolysis is for gelatinase, and Starch Hydrolysis is for amylase.

Answer:

C. Ferment sugars and reduce sulfur

Question 2