Question

please match each chemical with the role in plays in preparing dna for electrophoretic analysis.
1 nucleotide terminator 2 naoh 3 dna polymerase
match each of the options above to the items below.
splits the strands of double - stranded dna
replicates dna
halts dna synthesis at base - specific points

Explanation:

• NaOH: NaOH is a strong base. In the context of DNA, it can denature double - stranded DNA. Denaturation involves breaking the hydrogen bonds between the complementary base pairs of the two DNA strands, thus splitting the strands of double - stranded DNA.
• DNA polymerase: DNA polymerase is an enzyme that is crucial for DNA replication. It catalyzes the addition of deoxyribonucleotides to the growing DNA strand, using a pre - existing DNA strand as a template.
• Nucleotide terminator: Nucleotide terminators (such as dideoxynucleotides in Sanger sequencing) lack a 3' - OH group. When incorporated into a growing DNA strand by DNA polymerase, they prevent the further addition of nucleotides, thus halting DNA synthesis at base - specific points.

Answer:

• Splits the strands of double - stranded DNA: 2. NaOH
• Replicates DNA: 3. DNA polymerase
• Halts DNA synthesis at base - specific points: 1. Nucleotide terminator