Question

part 2: microscopy
a. steps to focus using a compound light microscope (fill in the blanks):

1. start with the ______ power objective lens.
2. place the slide on the ______ and secure it.
3. use the ______ adjustment knob to bring the object into rough focus.
4. switch to a ______ power lens for a closer view.
5. use the ______ adjustment knob to sharpen the image.
6. adjust the ______ to control light intensity.

Explanation:

1. When using a compound light microscope, we start with the low - power objective lens as it has a larger field of view, making it easier to locate the specimen.
2. The slide is placed on the stage of the microscope and secured with the stage clips.
3. The coarse adjustment knob is used for rough focusing at the beginning as it moves the stage (or the objective lens) more significantly to get the specimen approximately in focus.
4. After getting a rough focus with the low - power lens, we switch to a high - power lens to get a closer, more detailed view of the specimen.
5. The fine adjustment knob is used to sharpen the image (get a fine focus) because it moves the stage (or the objective lens) in very small increments.
6. The diaphragm (or iris diaphragm) is used to control the light intensity that passes through the specimen.

Answer:

1. low
2. stage
3. coarse
4. high
5. fine
6. diaphragm (or iris diaphragm)