QUESTION IMAGE
Question
in the oxidase test, what does a purple color indicate?
nitrate reduction
presence of catalase
presence of cytochrome c oxidase
acid production
question 4 (1 point)
in the gelatin hydrolysis test, a liquid medium after chilling indicates:
protein denaturation only
ph change
negative result
gelatinase positive
Brief Explanations
- Oxidase test: The oxidase test is used to determine if an organism produces cytochrome c oxidase. When cytochrome c oxidase is present, it reacts with the oxidase reagent (e.g., tetramethyl - p - phenylenediamine dihydrochloride) to form a purple - colored compound (indophenol).
- Gelatin hydrolysis test: Gelatin is a protein. Gelatinase is an enzyme that hydrolyzes gelatin. When gelatin is hydrolyzed, it cannot solidify (gel) upon chilling. A liquid medium after chilling indicates that gelatin has been hydrolyzed, which means the organism is gelatinase - positive.
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- For the oxidase test question: Presence of cytochrome c oxidase.
- For the gelatin hydrolysis test question: Gelatinase positive.