Question

7 multiple choice 10 points what is the correct order of the steps in polymerase chain reaction (pcr)? extension→denaturation→annealing extension→annealing→denaturation denaturation→annealing→extension annealing→denaturation→extension

Explanation:

PCR (Polymerase Chain Reaction) is a technique used to amplify DNA. The first step is denaturation, where the double - stranded DNA is heated to separate into single strands. Then, in annealing, the temperature is lowered to allow primers to bind to the complementary sequences on the single - stranded DNA. Finally, in extension, DNA polymerase synthesizes new DNA strands by adding nucleotides to the primers.

Answer:

Denaturation → Annealing → Extension