QUESTION IMAGE
Question
- given the following mrna, gua ccg uau gcu, what is the complementary dna template? what is the aa
Step1: Recall base - pairing rules
In DNA - mRNA base pairing, A (adenine) pairs with U (uracil) in mRNA, but in DNA - DNA (template - non - template strand), A pairs with T (thymine). C (cytosine) pairs with G (guanine) and vice - versa. Since we are going from mRNA to DNA template, we reverse the mRNA - DNA non - template (coding) strand pairing. The mRNA - DNA non - template strand pairing is A - U, U - A, C - G, G - C. And the DNA template - non - template strand pairing is A - T, T - A, C - G, G - C. So for mRNA to DNA template, it's A - T, U - A, C - G, G - C.
Step2: Apply base - pairing to each mRNA base
For the mRNA sequence GUA CCG UAU GCU:
- G in mRNA pairs with C in DNA template.
- U in mRNA pairs with A in DNA template.
- A in mRNA pairs with T in DNA template.
- C in mRNA pairs with G in DNA template.
- C in mRNA pairs with G in DNA template.
- G in mRNA pairs with C in DNA template.
- U in mRNA pairs with A in DNA template.
- A in mRNA pairs with T in DNA template.
- G in mRNA pairs with C in DNA template.
- C in mRNA pairs with G in DNA template.
- U in mRNA pairs with A in DNA template.
The DNA template sequence is CAT GGC ATA CGA.
Step3: Use the genetic code table
The genetic code table (codon - amino acid table) is used. The mRNA codons (read in groups of 3) are GUA (valine), CCG (proline), UAU (tyrosine), GCU (alanine).
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The DNA template sequence is CAT GGC ATA CGA and the amino - acid (AA) sequence is Valine - Proline - Tyrosine - Alanine.