Question

explain the process of tissue cultures using healthy cells.

Explanation:

Tissue culture with healthy cells involves several key steps. First, cells are isolated from a healthy tissue source. Then, they are placed in a sterile culture medium that provides nutrients, growth factors, and an appropriate environment (like controlled temperature and gas composition). The cells attach to the culture surface (if adherent) or remain suspended (if suspension culture). Over time, they grow and divide, forming a cell culture. This can be sub - cultured (split into new containers) to expand the cell population. Quality control measures like checking for contamination (bacteria, fungi, etc.) and maintaining cell viability (using techniques like trypan blue exclusion) are also part of the process.

Answer:

Tissue culture with healthy cells starts with cell isolation from a healthy tissue. Cells are placed in a sterile culture medium. They attach (if adherent) or remain suspended. Cells grow and divide. Sub - culturing may occur. Quality control (contamination check, viability test) is done.