Question

based on the banding patterns, how many sites tested negative for carp?
a. four
b. three
c. two
d. one

Explanation:

Step1: Identify negative banding pattern

A negative result for carp would be a banding pattern that does not match the positive control (assuming lane 8 could be a control or reference with a specific pattern for carp presence). If we assume that a certain band (like the lower - middle band in some of the lanes) is the indicator for carp presence.

Step2: Count lanes without the carp - specific band

Looking at lanes 3, 4, 7:

• Lane 3: has a band that might not be the carp - specific band (depending on the reference, but if we assume the carp - specific band is similar to lane 1,5,6 in the lower - middle region in a certain way).
• Lane 4: has a different banding pattern (the top - most band in lane 4 is not the carp - specific band if we assume the carp - specific band is in the lower part).
• Lane 7: has multiple bands, but if the carp - specific band is a single distinct band (say like in lane 1,5,6 in the lower - middle), then lane 7 does not have that specific band.

Answer:

B. Three