QUESTION IMAGE
Question
apply principles of building proteins to health
you are in a laboratory attempting to identify a genetic defect responsible for a disease. you believe you have located the gene that results in a faulty protein—but you arent sure!
how could you be sure you have located a section of dna that encodes for a protein?
write down the sequence to see what amino acids might be linked together.
allow the dna to be transcribed to rna and see what protein results.
compare the faulty protein to the dna.
- For the first option, writing down the DNA sequence to predict amino acids is not sufficient as it doesn't account for post - transcriptional and post - translational modifications.
- The second option is correct. DNA is first transcribed to RNA (mRNA), and then mRNA is translated into a protein. By allowing the DNA to be transcribed to RNA and observing the resulting protein, we can check if it matches the expected protein (faulty or not) based on the biological central dogma (DNA→RNA→protein).
- The third option of comparing the faulty protein directly to DNA is not straightforward. Proteins and DNA have different chemical structures and information representations. Proteins are made of amino acids, while DNA is made of nucleotides. There is no direct one - to - one comparison without going through the RNA intermediate.
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Second option: Allow the DNA to be transcribed to RNA and see what protein results.